BAIT

KNS1

serine/threonine protein kinase KNS1, L124, L000000910, YLL019C
Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

MMS22

SLM2, YLR320W
Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Differential phosphorylation of a regulatory subunit of protein kinase CK2 by target of rapamycin complex 1 signaling and the Cdc-like kinase Kns1.

Sanchez-Casalongue ME, Lee J, Diamond A, Shuldiner S, Moir RD, Willis IM

Transcriptional regulation of ribosome and tRNA synthesis plays a central role in determining protein synthetic capacity and is tightly controlled in response to nutrient availability and cellular stress. In Saccharomyces cerevisiae, the regulation of ribosome and tRNA synthesis was recently shown to involve the Cdc-like kinase Kns1 and the GSK-3 kinase Mck1. In this study, we explored additional roles for ... [more]

J. Biol. Chem. Mar. 13, 2015; 290(11);7221-33 [Pubmed: 25631054]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • SGA for rapamycin sensitivity using kns1 mck1 double mutant as bait
  • Table S1
  • hit shows rapamycin sensitivity in combination with either kns1 or mck1

Curated By

  • BioGRID