BAIT

CUL4B

CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2
cullin 4B
UBI
NEDD
GO Process (3)
GO Function (2)
GO Component (5)

External Database Linkouts

HGNC | OMIM | VEGA | Entrez Gene | RefSeq | UniprotKB | Ensembl | HPRD
Homo sapiens

PREY

DCAF4

WDR21, WDR21A
DDB1 and CUL4 associated factor 4
UBI
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

External Database Linkouts

VEGA | HGNC | Entrez Gene | RefSeq | UniprotKB | Ensembl | HPRD
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M

The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]

Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]

Throughput

  • High Throughput

Additional Notes

  • interaction detected by quantitative BAC-GFP interactomics (QUBIC)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DCAF4 CUL4B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9998BioGRID
2228432
DCAF4 CUL4B
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID