BAIT

RIT1

NS8, RIBB, RIT, ROC1, RP11-101O6.4
Ras-like without CAAX 1
GO Process (4)
GO Function (2)
GO Component (0)
Homo sapiens
PREY

RALGDS

RGDS, RGF, RalGEF, RP11-326L24.1
ral guanine nucleotide dissociation stimulator
GO Process (3)
GO Function (2)
GO Component (1)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Biochemical characterization of the Ras-related GTPases Rit and Rin.

Shao H, Kadono-Okuda K, Finlin BS, Andres DA

We report the biochemical characterization of Rit and Rin, two members of the Ras superfamily identified by expression cloning. Recombinant Rit and Rin bind GTP and exhibit intrinsic GTPase activity. Conversion of Gln to Leu at position 79 (for Rit) or 78 (for Rin) (equivalent to position 61 in Ras) resulted in a complete loss of GTPase activity. Surprisingly, significant ... [more]

Arch. Biochem. Biophys. Nov. 15, 1999; 371(2);207-19 [Pubmed: 10545207]

Throughput

  • Low Throughput

Curated By

  • BioGRID