BAIT

SSN3

CDK8, GIG2, NUT7, RYE5, SRB10, SSX7, UME5, URR1, cyclin-dependent serine/threonine protein kinase SSN3, L000002795, S000029631, L000002443, S000029520, L000002103, YPL042C
Cyclin-dependent protein kinase; component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
PHO
KIN

External Database Linkouts

SGD | Entrez Gene | RefSeq | UniprotKB
Saccharomyces cerevisiae (S288c)

PREY

PSY2

YNL201C
Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
UBI

External Database Linkouts

SGD | Entrez Gene | RefSeq | UniprotKB
Saccharomyces cerevisiae (S288c)

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Functional organization of the S. cerevisiae phosphorylation network.

Fiedler D, Braberg H, Mehta M, Chechik G, Cagney G, Mukherjee P, Silva AC, Shales M, Collins SR, van Wageningen S, Kemmeren P, Holstege FC, Weissman JS, Keogh MC, Koller D, Shokat KM, Krogan NJ

Reversible protein phosphorylation is a signaling mechanism involved in all cellular processes. To create a systems view of the signaling apparatus in budding yeast, we generated an epistatic miniarray profile (E-MAP) comprised of 100,000 pairwise, quantitative genetic interactions, including virtually all protein and small-molecule kinases and phosphatases as well as key cellular regulators. Quantitative genetic interaction mapping reveals factors working ... [more]

Cell Mar. 06, 2009; 136(5);952-63 [Pubmed: 19269370]

Quantitative Score

  • 2.150585 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size

Additional Notes

  • An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.0 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).

Curated By

  • BioGRID