BAIT

DBF2

serine/threonine-protein kinase DBF2, L000000487, YGR092W
Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
UBI
PHO

External Database Linkouts

SGD | Entrez Gene | RefSeq | UniprotKB
Saccharomyces cerevisiae (S288c)

PREY

TOF2

L000004245, YKR010C
Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
PHO
SUMO
GO Process (4)
GO Function (1)
GO Component (2)

External Database Linkouts

SGD | Entrez Gene | RefSeq | UniprotKB
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.

Waples WG, Chahwan C, Ciechonska M, Lavoie BD

The completion of chromosome segregation during anaphase requires the hypercondensation of the approximately 1-Mb rDNA array, a reaction dependent on condensin and Cdc14 phosphatase. Using systematic genetic screens, we identified 29 novel genetic interactions with budding yeast condensin. Of these, FOB1, CSM1, LRS4, and TOF2 were required for the mitotic condensation of the tandem rDNA array localized on chromosome XII. ... [more]

Mol. Biol. Cell Jan. 01, 2009; 20(1);245-55 [Pubmed: 18923139]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity

Curated By

  • BioGRID