PREY

ZTL

ADAGIO 1, ADO1, FKF1-LIKE PROTEIN 2, FKL2, LKP1, LOV KELCH PROTEIN 1, MSF19.2, MSF19_2, ZEITLUPE, AT5G57360
adagio protein 1
Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Partners in time: EARLY BIRD associates with ZEITLUPE and regulates the speed of the Arabidopsis clock.

Johansson M, McWatters HG, Bako L, Takata N, Gyula P, Hall A, Somers DE, Millar AJ, Eriksson ME

The circadian clock of the model plant Arabidopsis thaliana is made up of a complex series of interacting feedback loops whereby proteins regulate their own expression across day and night. early bird (ebi) is a circadian mutation that causes the clock to speed up: ebi plants have short circadian periods, early phase of clock gene expression and are early flowering. ... [more]

Unknown Feb. 07, 2011; 0(0); [Pubmed: 21300918]

Throughput

  • Low Throughput

Curated By

  • BioGRID