BAIT

UBP8

ubiquitin-specific protease UBP8, L000004587, YMR223W
Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B
GO Process (4)
GO Function (2)
GO Component (3)
Saccharomyces cerevisiae (S288c)
PREY

HTB2

histone H2B, L000000830, YBL002W
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability.

Chandrasekharan MB, Huang F, Sun ZW

The mechanism by which ubiquitination of histone H2B (H2Bub1) regulates H3-K4 and -K79 methylation and the histone H2A-H2B chaperone Spt16-mediated nucleosome dynamics during transcription is not fully understood. Upon investigating the effect of H2Bub1 on chromatin structure, we find that contrary to the supposed role for H2Bub1 in opening up chromatin, it is important for nucleosome stability. First, we show ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 29, 2009; 106(39);16686-91 [Pubmed: 19805358]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • (ubp8 ubp10 H2B-K123R triple mutant)
  • The slow growth defect of ubp8 ubp10 double mutants can be rescued by eliminating H2B (HTB1,HTB2) ubiquitination through the mutation of the site of ubiquitination (H2B-K123R).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBP8 HTB2
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
438819

Curated By

  • BioGRID