BAIT

SMP1

L000004146, YBR182C
MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

MSN2

stress-responsive transcriptional activator MSN2, L000001198, YMR037C
Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress
GO Process (22)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Prediction of putative protein interactions through evolutionary analysis of osmotic stress response in the model yeast Saccharomyces cerevisae.

Thorne TW, Ho HL, Huvet M, Haynes K, Stumpf MP

The osmotic stress response signalling pathway of the model yeast Saccharomyces cerevisae is crucial for the survival of cells under osmotic stress, and is preserved to varying degrees in other related fungal species. We apply a method for inference of ancestral states of characteristics over a phylogeny to 17 fungal species to infer the maximum likelihood estimate of presence or ... [more]

Fungal Genet. Biol. May. 01, 2011; 48(5);504-11 [Pubmed: 21193057]

Throughput

  • Low Throughput

Additional Notes

  • Directionality not explicit in paper

Curated By

  • BioGRID