BAIT

PRRC2A

BAT2, D6S51, D6S51E, G2, DADB-70P7.5
proline-rich coiled-coil 2A
GO Process (0)
GO Function (2)
GO Component (4)
Homo sapiens
PREY

QARS

GLNRS, MSCCA, PRO2195
glutaminyl-tRNA synthetase
GO Process (4)
GO Function (2)
GO Component (3)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.

Lehner B, Semple JI, Brown SE, Counsell D, Campbell RD, Sanderson CM

High-throughput (HTP) protein-interaction assays, such as the yeast two-hybrid (Y2H) system, are enormously useful in predicting the functions of novel gene-products. HTP-Y2H screens typically do not include all of the reconfirmation and specificity tests used in small-scale studies, but the effects of omitting these steps have not been assessed. We performed HTP-Y2H screens that included all standard controls, using the ... [more]

Genomics Jan. 01, 2004; 83(1);153-67 [Pubmed: 14667819]

Throughput

  • Low Throughput

Curated By

  • BioGRID