Y32H12A.4, CELE_Y32H12A.4

szy-2 encodes an ortholog of various protein phosphatase 1 (PP1) regulatory (inhibitor) subunit 2 proteins; the phosphatase inhibitory activity of recombinant SZY-2 has been validated in vitro, with an IC50 of 5 nM versus purified PP1 catalytic subunit; SZY-2 has anomalously low mobility in SDS-PAGE, consistent with the hypothesis that it (like its orthologs) is a naturally disordered protein that uses native flexibility to bind its substrates; SZY-2 is efficiently phosphorylated in vitro by casein kinase II or CDC2/cyclinB, but not by glycogen synthase kinase-3; szy-2 is required for embryonic development in mass RNAi assays.

40968

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