CELE_F22B7.13, ags-3.2, F22B7.13

gpr-1 encodes an extremely similar (97% identity) paralog of GPR-2; GPR-1 (and GPR-2) proteins have two N-terminal tetratricopeptide-like motifs and a C-terminal GoLoco/GPR (G protein regulatory) motif, the latter of which has also been found in mammalian AGS3 and Drosophila Pins; GPR-1 is required for normally asymmetrical cleavage of one-cell embryos; GPR-1 and GPR-2 form a high molecular weight (~700 kDa) complex that includes LIN-5; GPR-1 binds GDP-bound GOA-1 via a GoLoco/GPR motif, and depends on RIC-8 for this binding; GPR-1/2, GOA-1, and LIN-5 colocalize at the cortex of early embryos; cortical enrichment of GPR-1 requires LIN-5, PAR-2, PAR-3, and LET-99; the asymmetric distribution of GPR-1/2 and LET-99 in EMS cells is dependent on MES-1/SRC-1 signaling; GPR-1/2 is co-immunoprecipitated with RIC-8 and GPA-16.

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