BAIT

CSN5B

AJH2, COP9-signalosome 5B, CSN5, F3I17.12, F3I17_12, AT1G71230
COP9 signalosome complex subunit 5b
Arabidopsis thaliana (Columbia)
PREY

CSN6B

COP9 signalosome subunit 6B, M3E9.140, M3E9_140, AT4G26430
COP9 signalosome complex subunit 6b
GO Process (4)
GO Function (0)
GO Component (2)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Subunit interaction maps for the regulatory particle of the 26S proteasome and the COP9 signalosome.

Fu H, Reis N, Lee Y, Glickman MH, Vierstra RD

The 26S proteasome plays a major role in eukaryotic protein breakdown, especially for ubiquitin-tagged proteins. Substrate specificity is conferred by the regulatory particle (RP), which can dissociate into stable lid and base subcomplexes. To help define the molecular organization of the RP, we tested all possible paired interactions among subunits from Saccharomyces cerevisiae by yeast two-hybrid analysis. Within the base, ... [more]

EMBO J. Dec. 17, 2001; 20(24);7096-107 [Pubmed: 11742986]

Throughput

  • Low Throughput

Curated By

  • BioGRID