PREY

RAC1

Rac-1, TC-25, p21-Rac1, MIG5
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GO Process (41)
GO Function (6)
GO Component (10)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

AMPylation of Rho GTPases subverts multiple host signaling processes.

Woolery AR, Yu X, LaBaer J, Orth K

Rho GTPases are frequent targets of virulence factors as they are keystone signaling molecules. Herein, we demonstrate that AMPylation of Rho GTPases by VopS is a multifaceted virulence mechanism that counters several host immunity strategies. Activation of NFκB, Erk and JNK kinase signaling pathways were inhibited in a VopS dependent manner during infection with Vibrio parahaemolyticus. Phosphorylation and degradation of ... [more]

J. Biol. Chem. Oct. 09, 2014; 0(0); [Pubmed: 25301945]

Throughput

  • High Throughput

Additional Notes

  • figure 6. NAPPA high-through screen.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
C1QA RAC1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1034526

Curated By

  • BioGRID