Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.

Butland SL, Sanders SS, Schmidt ME, Riechers SP, Lin DT, Martin DD, Vaid K, Graham RK, Singaraja RR, Wanker EE, Conibear E, Hayden MR

HIP14 is the most highly conserved of 23 human palmitoyl acyltransferases (PATs) that catalyze the post-translational addition of palmitate to proteins, including huntingtin (HTT). HIP14 is dysfunctional in the presence of mutant HTT (mHTT), the causative gene for Huntington disease (HD), and we hypothesize that reduced palmitoylation of HTT and other HIP14 substrates contributes to the pathogenesis of the disease. ... [more]

Hum. Mol. Genet. Aug. 01, 2014; 23(15);4142-60 [Pubmed: 24705354]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FAM135B ZDHHC17	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	0.9976	BioGRID	1194110
FAM135B ZDHHC17	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9774	BioGRID	2245779
FAM135B ZDHHC17	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9463	BioGRID	3065063

Curated By

BioGRID

Interaction Summary

ZDHHC17

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]magnesium ion transmembrane transporter activity [IDA]palmitoyltransferase activity [IDA]protein binding [IPI]protein-cysteine S-palmitoyltransferase activity [IDA]signal transducer activity [IMP]

Gene Ontology Cellular Component

FAM135B

Two-hybrid

Publication

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.

Throughput

Related interactions

Curated By

identical protein binding [IPI]
magnesium ion transmembrane transporter activity [IDA]
palmitoyltransferase activity [IDA]
protein binding [IPI]
protein-cysteine S-palmitoyltransferase activity [IDA]
signal transducer activity [IMP]