BAIT

PEX19-2

AtPEX19-2, K10A8.30, K10A8_30, peroxin 19-2, AT5G17550
peroxin 19-2
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

PXA1

ACN2, AtABCD1, COMATOSE, CTS, PED3, PEROXISOME DEFECTIVE 3, T5J17.20, T5J17_20, acetate non-utilizing 2, peroxisomal ABC transporter 1, AT4G39850
ABC transporter D family member 1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity.

Nyathi Y, Zhang X, Baldwin JM, Bernhardt K, Johnson B, Baldwin SA, Theodoulou FL, Baker A

Peroxisomal ABC transporters of animals and fungi are "half-size" proteins which dimerise to form a functional transporter. However, peroxisomal ABC transporters of land plants are synthesised as a single polypeptide which represents a fused heterodimer. The N- and C-terminal pseudo-halves of COMATOSE (CTS; AtABCD1) were expressed as separate polypeptides which bound Pex19 in vitro and targeted independently to the peroxisome ... [more]

FEBS Lett. Jul. 30, 2012; 586(16);2280-6 [Pubmed: 22710125]

Throughput

  • Low Throughput

Curated By

  • BioGRID