A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Endopolyploidization and flowering time are antagonistically regulated by checkpoint component MAD1 and immunity modulator MOS1.

Bao Z, Zhang N, Hua J

The spindle assembly checkpoint complex (SAC) is essential for quality control during mitosis in yeast and animals. However, its function in plants is not well understood. Here we show that MAD1, an Arabidopsis SAC component, is involved in endopolyploidization and flowering time via genetic interaction with MOS1, a negative regulator of plant immunity. MOS1 is found to interact with MAD2, ... [more]

Nat Commun Nov. 29, 2014; 5(0);5628 [Pubmed: 25429892]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT5G49880 SUF4	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Bao Z (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SUF4

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]protein binding [IPI]protein heterodimerization activity [IPI]protein homodimerization activity [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

AT5G49880