BAIT

TED6

F28H19.19, F28H19_19, tracheary element differentiation-related 6, AT1G43790
tracheary element differentiation-related 6 protein
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

IRX3

ATCESA7, CELLULOSE SYNTHASE CATALYTIC SUBUNIT, CELLULOSE SYNTHASE CATALYTIC SUBUNIT 7, CESA7, IRREGULAR XYLEM 3, MUR10, MURUS 10, T10B6.80, T10B6_80, AT5G17420
cellulose synthase A catalytic subunit 7 [UDP-forming]
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Identifying new components participating in the secondary cell wall formation of vessel elements in zinnia and Arabidopsis.

Endo S, Pesquet E, Yamaguchi M, Tashiro G, Sato M, Toyooka K, Nishikubo N, Udagawa-Motose M, Kubo M, Fukuda H, Demura T

Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell ... [more]

Plant Cell Apr. 01, 2009; 21(4);1155-65 [Pubmed: 19383897]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TED6 IRX3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
TED6 IRX3
Co-purification
Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Low-BioGRID
-

Curated By

  • BioGRID