BAIT

RAP2.11

T29J13.210, T29J13_210, related to AP2 11, AT5G19790
ethylene-responsive transcription factor RAP2-11
Arabidopsis thaliana (Columbia)
PREY

HY5

BZIP TRANSCRIPTION FACTOR HY5, ELONGATED HYPOCOTYL 5, F2I11.150, F2I11_150, REVERSAL OF THE DET PHENOTYPE 5, TED 5, AT5G11260
transcription factor HY5
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors.

Gong W, He K, Covington M, Dinesh-Kumar SP, Snyder M, Harmer SL, Zhu YX, Deng XW

We used our collection of Arabidopsis transcription factor (TF) ORFeome clones to construct protein microarrays containing as many as 802 TF proteins. These protein microarrays were used for both protein-DNA and protein-protein interaction analyses. For protein-DNA interaction studies, we examined AP2/ERF family TFs and their cognate cis-elements. By careful comparison of the DNA-binding specificity of 13 TFs on the protein ... [more]

Mol Plant Jan. 01, 2008; 1(1);27-41 [Pubmed: 19802365]

Throughput

  • High Throughput

Additional Notes

  • Table 3
  • protein microarray

Curated By

  • BioGRID