BAIT

ARF6

T4K22.6, T4K22_6, auxin response factor 6, AT1G30330
auxin response factor 6
GO Process (3)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

IAA19

MASSUGU 2, MJK13.22, MSG2, indole-3-acetic acid inducible 19, AT3G15540
auxin-responsive protein IAA19
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis.

Piya S, Shrestha SK, Binder B, Stewart CN, Hewezi T

The phytohormone auxin regulates nearly all aspects of plant growth and development. Based on the current model in Arabidopsis thaliana, Auxin/indole-3-acetic acid (Aux/IAA) proteins repress auxin-inducible genes by inhibiting auxin response transcription factors (ARFs). Experimental evidence suggests that heterodimerization between Aux/IAA and ARF proteins are related to their unique biological functions. The objective of this study was to generate the ... [more]

Front Plant Sci Jan. 08, 2015; 5(0);744 [Pubmed: 25566309]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IAA19 ARF6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID