A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis.

Piya S, Shrestha SK, Binder B, Stewart CN, Hewezi T

The phytohormone auxin regulates nearly all aspects of plant growth and development. Based on the current model in Arabidopsis thaliana, Auxin/indole-3-acetic acid (Aux/IAA) proteins repress auxin-inducible genes by inhibiting auxin response transcription factors (ARFs). Experimental evidence suggests that heterodimerization between Aux/IAA and ARF proteins are related to their unique biological functions. The objective of this study was to generate the ... [more]

Front Plant Sci Jan. 08, 2015; 5(0);744 [Pubmed: 25566309]

Throughput

Low Throughput

Additional Notes

Figure 2
bimolecular fluorescence complementation (BiFC) assays

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MP IAA34	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Piya S (2015)	High	-	BioGRID	-
MP IAA34	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Vernoux T (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MP

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS, TAS]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

IAA34