BAIT

AT4G23950

T32A16.120, T32A16_120
Galactose-binding protein
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AT1G22882

Galactose-binding protein
GO Process (0)
GO Function (0)
GO Component (3)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Characterization of two distinct subfamilies of SUN-domain proteins in Arabidopsis and their interactions with the novel KASH-domain protein AtTIK.

Graumann K, Vanrobays E, Tutois S, Probst AV, Evans DE, Tatout C

SUN-domain proteins belong to a gene family including classical Cter-SUN and mid-SUN subfamilies differentiated by the position of the SUN domain within the protein. Although present in animal and plant species, mid-SUN proteins have so far remained poorly described. Here, we used a combination of genetics, yeast two-hybrid and in planta transient expression methods to better characterize the SUN family ... [more]

J. Exp. Bot. Dec. 01, 2014; 65(22);6499-512 [Pubmed: 25217773]

Throughput

  • Low Throughput

Additional Notes

  • Figure 3
  • split-ubiquitin assay

Curated By

  • BioGRID