BAIT

TUB1

F10A5.3, F10A5_3, tubulin beta-1 chain, AT1G75780
tubulin beta-1 chain
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

GRF5

F1N13.190, F1N13_190, GF14 UPSILON, GF14 UPSILON CHAIN, general regulatory factor 5, AT5G16050
14-3-3-like protein GF14 upsilon
GO Process (1)
GO Function (2)
GO Component (8)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interaction of a 14-3-3 protein with the plant microtubule-associated protein EDE1.

Pignocchi C, Doonan JH

The cell cycle-regulated protein ENDOSPERM DEFECTIVE 1 (EDE1) is a novel plant microtubule-associated protein essential for plant cell division and for microtubule organization in endosperm. EDE1 is only present on microtubules at mitosis and its expression is highly cell cycle regulated both at the protein and the transcript levels.To search for EDE1-interacting proteins, a yeast two-hybrid screen was used in ... [more]

Ann. Bot. May. 01, 2011; 107(7);1103-9 [Pubmed: 21558460]

Throughput

  • Low Throughput

Curated By

  • BioGRID