AT1G62740
Gene Ontology Biological Process
HSP81-2
Gene Ontology Biological Process
- chaperone-mediated protein complex assembly [IMP]
- defense response [IMP]
- defense response to bacterium, incompatible interaction [IMP]
- flower development [IMP]
- heat acclimation [IEP]
- leaf development [IMP]
- negative regulation of seed germination [IMP]
- protein folding [ISS]
- protein stabilization [IMP]
- response to heat [IEP]
- response to salt stress [IMP]
- response to water deprivation [IMP]
- stomatal closure [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Study of receptor-chaperone interactions using the optical technique of spectroscopic ellipsometry.
This work describes a detailed quantitative interaction study between the novel plastidial chaperone receptor OEP61 and isoforms of the chaperone types Hsp70 and Hsp90 using the optical method of total internal reflection ellipsometry (TIRE). The receptor OEP61 was electrostatically immobilized on a gold surface via an intermediate layer of polycations. The TIRE measurements allowed the evaluation of thickness changes in ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID