CPL1
Gene Ontology Biological Process
Gene Ontology Molecular Function
HYL1
Gene Ontology Biological Process
- leaf proximal/distal pattern formation [IMP]
- leaf vascular tissue pattern formation [IMP]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- pre-miRNA processing [IMP]
- production of miRNAs involved in gene silencing by miRNA [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- response to abscisic acid [IMP]
- response to auxin [IMP]
- response to cytokinin [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1.
MicroRNAs (miRNAs) are processed from primary transcripts that contain partially self-complementary foldbacks. As in animals, the core microprocessor in plants is a Dicer protein, DICER-LIKE1 (DCL1). Processing accuracy and strand selection is greatly enhanced through the RNA binding protein HYPONASTIC LEAVES 1 (HYL1) and the zinc finger protein SERRATE (SE). We have combined a luciferase-based genetic screen with whole-genome sequencing ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC
- Figure 3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CPL1 HYL1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1112574 |
Curated By
- BioGRID