BAIT

ABF2

ABSCISIC ACID RESPONSIVE ELEMENTS-BINDING PROTEIN 2, AREB1, ATAREB1, abscisic acid responsive elements-binding factor 2, AT1G45249
abscisic acid responsive elements-binding factor 2
Arabidopsis thaliana (Columbia)
PREY

ABF4

ABA-RESPONSIVE ELEMENT BINDING PROTEIN 2, ABRE binding factor 4, AREB2, MVI11.7, AT3G19290
ABRE binding factor 4
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation.

Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K

A myriad of drought stress-inducible genes have been reported, and many of these are activated by abscisic acid (ABA). In the promoter regions of such ABA-regulated genes, conserved cis-elements, designated ABA-responsive elements (ABREs), control gene expression via bZIP-type AREB/ABF transcription factors. Although all three members of the AREB/ABF subfamily, AREB1, AREB2, and ABF3, are upregulated by ABA and water stress, ... [more]

Plant J. Feb. 01, 2010; 61(4);672-85 [Pubmed: 19947981]

Throughput

  • Low Throughput

Curated By

  • BioGRID