BAIT

TUA5

ALPHA TUBULIN, T29J13.200, tubulin alpha-5, AT5G19780
tubulin alpha-5
GO Process (2)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

MAP65-2

F10M23.100, F10M23_100, microtubule-associated protein 65-2, AT4G26760
microtubule-associated protein 65-2
GO Process (3)
GO Function (0)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cellulose synthase interactive protein 1 (CSI1) links microtubules and cellulose synthase complexes.

Li S, Lei L, Somerville CR, Gu Y

Cellulose synthase (CESA) complexes can be observed by live-cell imaging to move with trajectories that parallel the underlying cortical microtubules. Here we report that CESA interactive protein 1 (CSI1) is a microtubule-associated protein that bridges CESA complexes and cortical microtubules. Simultaneous in vivo imaging of CSI1, CESA complexes, and microtubules demonstrates that the association of CESA complexes and cortical microtubules ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 03, 2012; 109(1);185-90 [Pubmed: 22190487]

Throughput

  • Low Throughput

Curated By

  • BioGRID