BAIT

ACT2

DEFORMED ROOT HAIRS 1, DER1, ENHANCER OF LRX1 2, ENL2, LIGHT STRESS-REGULATED 2, LSR2, actin 2, AT3G18780
actin 2
Arabidopsis thaliana (Columbia)
PREY

FIM1

ARABIDOPSIS THALIANA FIMBRIN 1, ATFIM1, F10M23.40, F10M23_40, FIMBRIN-LIKE PROTEIN, fimbrin 1, AT4G26700
fimbrin 1
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors.

Gehl C, Kaufholdt D, Hamisch D, Bikker R, Kudla J, Mendel RR, Haensch R

Dynamic protein-protein interactions are essential in all cellular and developmental processes. Protein-fragment complementation assays allow such protein-protein interactions to be investigated in vivo. In contrast to other protein-fragment complementation assays, the split-luciferase (split-LUC) complementation approach facilitates dynamic and quantitative in vivo analysis of protein interactions, as the restoration of luciferase activity upon protein-protein interaction of investigated proteins is reversible. Here, ... [more]

Plant J. Aug. 01, 2011; 67(3);542-53 [Pubmed: 21481030]

Throughput

  • Low Throughput

Additional Notes

  • FLuCI

Curated By

  • BioGRID