BAIT

OFP1

ARABIDOPSIS THALIANA OVATE FAMILY PROTEIN 1, ATOFP1, T20L15.110, T20L15_110, ovate family protein 1, AT5G01840
ovate family protein 1
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

KU70

ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, F6I1.2, F6I1_2, KU70 homolog, KU70-LIKE PROTEIN, AT1G16970
ATP-dependent DNA helicase 2 subunit KU70
GO Process (3)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Ovate family protein 1 as a plant Ku70 interacting protein involving in DNA double-strand break repair.

Wang YK, Chang WC, Liu PF, Hsiao MK, Lin CT, Lin SM, Pan RL

The Ku heterodimer, a DNA repair protein complex consisting of 70- and 80-kDa subunits, is involved in the non-homologous end-joining (NHEJ) pathway. Plants are thought to use the NHEJ pathway primarily for the repair of DNA double-strand breaks (DSBs). The Ku70/80 protein has been identified in many plants and been shown to possess several similar functions to its counter protein ... [more]

Plant Mol. Biol. Nov. 01, 2010; 74(4);453-66 [Pubmed: 20844935]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KU70 OFP1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID