A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities.

Geiger D, Scherzer S, Mumm P, Marten I, Ache P, Matschi S, Liese A, Wellmann C, Al-Rasheid KA, Grill E, Romeis T, Hedrich R

In response to drought stress, the phytohormone abscisic acid (ABA) induces stomatal closure. Thereby the stress hormone activates guard cell anion channels in a calcium-dependent, as well as -independent, manner. Open stomata 1 protein kinase (OST1) and ABI1 protein phosphatase (ABA insensitive 1) represent key components of calcium-independent ABA signaling. Recently, the guard cell anion channel SLAC1 was identified. When ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 27, 2010; 107(17);8023-8 [Pubmed: 20385816]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CPK23

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin-dependent protein kinase activity [ISS]kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]protein phosphatase binding [IPI]

Gene Ontology Cellular Component

ABI1