BAIT

ACT1

AAc1, ARABIDOPSIS ACTIN 1, F13M22.12, F13M22_12, actin 1, AT2G37620
actin 1
GO Process (3)
GO Function (1)
GO Component (6)
Arabidopsis thaliana (Columbia)
PREY

ANN5

ANNAT5, ANNEXIN ARABIDOPSIS THALIANA 5, T23K23.6, T23K23_6, annexin 5, AT1G68090
annexin D5
GO Process (5)
GO Function (2)
GO Component (0)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Annexin5 plays a vital role in Arabidopsis pollen development via Ca2+-dependent membrane trafficking.

Zhu J, Wu X, Yuan S, Qian D, Nan Q, An L, Xiang Y

The regulation of pollen development and pollen tube growth is a complicated biological process that is crucial for sexual reproduction in flowering plants. Annexins are widely distributed from protists to higher eukaryotes and play multiple roles in numerous cellular events by acting as a putative "linker" between Ca2+ signaling, the actin cytoskeleton and the membrane, which are required for pollen ... [more]

PLoS ONE Jul. 16, 2014; 9(7);e102407 [Pubmed: 25019283]

Throughput

  • Low Throughput

Curated By

  • BioGRID