An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Light control of plasma membrane recruitment using the Phy-PIF system.

Toettcher JE, Gong D, Lim WA, Weiner OD

The ability to control the activity of intracellular signaling processes in live cells would be an extraordinarily powerful tool. Ideally, such an intracellular input would be (i) genetically encoded, (ii) able to be turned on and off in defined temporal or spatial patterns, (iii) fast to switch between on and off states, and (iv) orthogonal to other cellular processes. The ... [more]

Meth. Enzymol. May. 24, 2011; 497(0);409-23 [Pubmed: 21601096]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PHYB

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled photoreceptor activity [ISS]far-red light photoreceptor activity [IDA]identical protein binding [IPI]protein binding [IPI]protein histidine kinase activity [ISS]red light photoreceptor activity [IDA]red or far-red light photoreceptor activity [IMP]signal transducer activity [ISS]

Gene Ontology Cellular Component

PIL2