BAIT

ARC5

ACCUMULATION AND REPLICATION OF CHLOROPLAST 5, DRP5B, Dynamin related protein 5B, AT3G19720
dynamin-like protein ARC5
GO Process (3)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

PDV2

F7H1.9, F7H1_9, PLASTID DIVISION2, AT2G16070
plastid division protein 2
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Plastid division control: the PDV proteins regulate DRP5B dynamin activity.

Holtsmark I, Lee S, Lunde KA, Auestad K, Maple-Grodem J, Moller SG

Chloroplast division represents a fundamental but complex biological process involving remnants of the ancestral bacterial division machinery and proteins of eukaryotic origin. Moreover, the chloroplast division machinery is divided into stromal and cytosolic sub machineries, which coordinate and control their activities to ensure appropriate division initiation and progression. Dynamin related protein 5B (DRP5B) and plastid division protein 1 and 2 ... [more]

Plant Mol. Biol. Jun. 01, 2013; 82(3);255-66 [Pubmed: 23595201]

Throughput

  • Low Throughput

Additional Notes

  • Figure 3
  • bimolecular fluorescence complementation (BiFC)

Curated By

  • BioGRID