BAIT

HYL1

DRB1, DSRNA-BINDING PROTEIN 1, F21M12.9, F21M12_9, HYPONASTIC LEAVES 1, AT1G09700
double-stranded RNA-binding protein 1
Arabidopsis thaliana (Columbia)
PREY

VOZ1

ATVOZ1, F3M18.4, F3M18_4, VASCULAR PLANT ONE ZINC FINGER PROTEIN, AT1G28520
vascular plant one zinc finger protein
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA.

Yang X, Ren W, Zhao Q, Zhang P, Wu F, He Y

MicroRNA (miRNA) plays an important role in the control of gene expression. HYPONASTIC LEAVES1 (HYL1) is a double-stranded RNA-binding protein that forms a complex with DICER-LIKE1 (DCL1) and SERRATE (SE) to process primary miRNA (pri-miRNA) into mature miRNA. Although HYL1 has been shown to partner with DCL1 to enhance miRNA accuracy, the mechanism by which HYL1 selects the DCL1-targeted cleavage ... [more]

Nucleic Acids Res. Oct. 29, 2014; 42(19);12224-36 [Pubmed: 25294831]

Throughput

  • Low Throughput

Curated By

  • BioGRID