BAIT

SUN1

ARABIDOPSIS SAD1/UNC-84 DOMAIN PROTEIN 1, ATSUN1, MUG13.15, MUG13_15, SAD1/UNC-84 domain protein 1, AT5G04990
SAD1/UNC-84 domain protein 1
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SUN2

ARABIDOPSIS SAD1/UNC-84 DOMAIN PROTEIN 2, ATSUN2, SAD1/UNC-84 domain protein 2, AT3G10730
SAD1/UNC-84 domain-containing protein 2
GO Process (2)
GO Function (1)
GO Component (5)

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The kinesin AtPSS1 promotes synapsis and is required for proper crossover distribution in meiosis.

Duroc Y, Lemhemdi A, Larcheveque C, Hurel A, Cuacos M, Cromer L, Horlow C, Armstrong SJ, Chelysheva L, Mercier R

Meiotic crossovers (COs) shape genetic diversity by mixing homologous chromosomes at each generation. CO distribution is a highly regulated process. CO assurance forces the occurrence of at least one obligatory CO per chromosome pair, CO homeostasis smoothes out the number of COs when faced with variation in precursor number and CO interference keeps multiple COs away from each other along ... [more]

PLoS Genet. Oct. 01, 2014; 10(10);e1004674 [Pubmed: 25330379]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SUN1 SUN2
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-
SUN1 SUN2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
1111903
SUN2 SUN1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
1111906

Curated By

  • BioGRID