BAIT

AT3G16270

MYA6.8
ENTH/VHS family protein
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

AT3G11130

F11B9.30
Clathrin, heavy chain
GO Process (1)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network.

Sauer M, Delgadillo MO, Zouhar J, Reynolds GD, Pennington JG, Jiang L, Liljegren SJ, Stierhof YD, De Jaeger G, Otegui MS, Bednarek SY, Rojo E

Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants that secrete the ... [more]

Plant Cell Jun. 01, 2013; 25(6);2217-35 [Pubmed: 23771894]

Throughput

  • Low Throughput

Curated By

  • BioGRID