BAIT

NTRC

NADPH-dependent thioredoxin reductase C, T32G6.20, T32G6_20, AT2G41680
NADPH-dependent thioredoxin reductase C
Arabidopsis thaliana (Columbia)
PREY

THX

ATHX, ATX, THIOREDOXIN X, AT1G50320
thioredoxin X
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Molecular recognition in the interaction of chloroplast 2-Cys peroxiredoxin with NADPH-thioredoxin reductase C (NTRC) and thioredoxin x.

Bernal-Bayard P, Ojeda V, Hervas M, Cejudo FJ, Navarro JA, Velazquez-Campoy A, Perez-Ruiz JM

In addition to the standard NADPH thioredoxin reductases (NTRs), plants hold a plastidic NTR (NTRC), with a thioredoxin module fused at the C-terminus. NTRC is an efficient reductant of 2-Cys peroxiredoxins (2-Cys Prxs). The interaction of NTRC and chloroplastic thioredoxin x with 2-Cys Prxs has been confirmed in vivo, by bimolecular fluorescence complementation (BiFC) assays, and in vitro, by isothermal ... [more]

FEBS Lett. Nov. 28, 2014; 588(23);4342-7 [Pubmed: 25448674]

Throughput

  • Low Throughput

Additional Notes

  • BiFC assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NTRC THX
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID