An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Protein phosphatase 2A (PP2A) regulatory subunit B'Î³ interacts with cytoplasmic ACONITASE 3 and modulates the abundance of AOX1A and AOX1D in Arabidopsis thaliana.

Konert G, Trotta A, Kouvonen P, Rahikainen M, Durian G, Blokhina O, Fagerstedt K, Muth D, Corthals GL, Kangasjaervi S

Organellar reactive oxygen species (ROS) signalling is a key mechanism that promotes the onset of defensive measures in stress-exposed plants. The underlying molecular mechanisms and feedback regulation loops, however, still remain poorly understood. Our previous work has shown that a specific regulatory B'Î³ subunit of protein phosphatase 2A (PP2A) is required to control organellar ROS signalling and associated metabolic adjustments ... [more]

New Phytol. Feb. 01, 2015; 205(3);1250-63 [Pubmed: 25307043]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ATB' GAMMA ACO3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Konert G (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATB' GAMMA

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(U) RNA binding [IDA]protein phosphatase type 2A regulator activity [ISS]

Gene Ontology Cellular Component

ACO3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]aconitate hydratase activity [IMP, ISS]copper ion binding [IDA]