Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes.

Hiltscher H, Rudnik R, Shaikhali J, Heiber I, Mellenthin M, Meirelles Duarte I, Schuster G, Kahmann U, Baier M

The rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-induced cell death 1; At1g32230) demonstrating that a major factor involved in redox-regulation genes for chloroplast antioxidant enzymes and protection against photooxidative stress, RIMB1, is identical to the regulator of disease response reactions and cell death, RCD1. Discovering this link let to our investigation of its regulatory mechanism. ... [more]

Front Plant Sci Oct. 09, 2014; 5(0);475 [Pubmed: 25295044]

Throughput

  • Low Throughput

Curated By

  • BioGRID