BAIT

RGA1

F2I9.19, F2I9_19, REPRESSOR OF GA, REPRESSOR OF GA1-3 1, RGA, AT2G01570
DELLA protein RGA
Arabidopsis thaliana (Columbia)
PREY

JKD

F15A17.180, F15A17_180, JACKDAW, AT5G03150
zinc finger protein JACKDAW
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

DELLA protein functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins.

Yoshida H, Hirano K, Sato T, Mitsuda N, Nomoto M, Maeo K, Koketsu E, Mitani R, Kawamura M, Ishiguro S, Tada Y, Ohme-Takagi M, Matsuoka M, Ueguchi-Tanaka M

DELLA protein is a key negative regulator of gibberellin (GA) signaling. Although how DELLA regulates downstream gene expression remains unclear, DELLA has been proposed to function as a transcriptional activator. However, because DELLA lacks a DNA-binding domain, intermediate protein(s) mediating the DELLA/DNA interaction are believed to be necessary for activating DELLA target genes. Here, using yeast hybrid screenings, we identified ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 12, 2014; 0(0); [Pubmed: 24821766]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RGA1 JKD
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
1173376

Curated By

  • BioGRID