BAIT

AT3G54500

hypothetical protein
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

LCL1

F9G14.150, F9G14_150, LHY/CCA1-like 1, AT5G02840
protein LHY/CCA1-like 1
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis circadian oscillator.

Xie Q, Wang P, Liu X, Yuan L, Wang L, Zhang C, Li Y, Xing H, Zhi L, Yue Z, Zhao C, McClung CR, Xu X

Transcriptional feedback loops are central to the architecture of eukaryotic circadian clocks. Models of the Arabidopsis thaliana circadian clock have emphasized transcriptional repressors, but recently, Myb-like REVEILLE (RVE) transcription factors have been established as transcriptional activators of central clock components, including PSEUDO-RESPONSE REGULATOR5 (PRR5) and TIMING OF CAB EXPRESSION1 (TOC1). We show here that NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED1 (LNK1) and ... [more]

Plant Cell Jul. 01, 2014; 26(7);2843-57 [Pubmed: 25012192]

Throughput

  • Low Throughput

Additional Notes

  • BiFC

Curated By

  • BioGRID