BAIT

GRF3

GF14PSI ISOFORM, MXI10.21, MXI10_21, RCI1, general regulatory factor 3, AT5G38480
14-3-3-like protein GF14 psi
GO Process (0)
GO Function (2)
GO Component (9)
Arabidopsis thaliana (Columbia)
PREY

ACS6

1-aminocyclopropane-1-carboxylic acid (acc) synthase 6, ATACS6, F8L21.70, F8L21_70, AT4G11280
1-aminocyclopropane-1-carboxylate synthase 6
Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

The Arabidopsis 14-3-3 protein RARE COLD INDUCIBLE 1A links low-temperature response and ethylene biosynthesis to regulate freezing tolerance and cold acclimation.

Catala R, Lopez-Cobollo R, Mar Castellano M, Angosto T, Alonso JM, Ecker JR, Salinas J

In plants, the expression of 14-3-3 genes reacts to various adverse environmental conditions, including cold, high salt, and drought. Although these results suggest that 14-3-3 proteins have the potential to regulate plant responses to abiotic stresses, their role in such responses remains poorly understood. Previously, we showed that the RARE COLD INDUCIBLE 1A (RCI1A) gene encodes the 14-3-3 psi isoform. ... [more]

Plant Cell Aug. 01, 2014; 26(8);3326-42 [Pubmed: 25122152]

Throughput

  • Low Throughput

Additional Notes

  • BiFC assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GRF3 ACS6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID