BAIT

CPK32

ATCPK32, CDPK32, calcium-dependent protein kinase 32, AT3G57530
calcium-dependent protein kinase 32
Arabidopsis thaliana (Columbia)
PREY

CNGC18

ATCNGC18, CYCLIC NUCLEOTIDE-GATED CHANNEL 18, T9L3.170, T9L3_170, AT5G14870
cyclic nucleotide-gated channel 18
GO Process (2)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

A calcium-dependent protein kinase interacts with and activates a calcium channel to regulate pollen tube growth.

Zhou L, Lan W, Jiang Y, Fang W, Luan S

Calcium, as a ubiquitous second messenger, plays essential roles in tip-growing cells, such as animal neurons, plant pollen tubes, and root hairs. However, little is known concerning the regulatory mechanisms that code and decode Ca(2+) signals in plants. The evidence presented here indicates that a calcium-dependent protein kinase, CPK32, controls polar growth of pollen tubes. Overexpression of CPK32 disrupted the ... [more]

Mol Plant Feb. 01, 2014; 7(2);369-76 [Pubmed: 24121288]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CPK32 CNGC18
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID