BAIT
HLA-DPA1
DP(W3), DP(W4), HLA-DP1A, HLADP, HLASB, PLT1, DAAP-277G18.1
major histocompatibility complex, class II, DP alpha 1
GO Process (10)
GO Function (2)
GO Component (12)
Gene Ontology Biological Process
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP, TAS]
- cellular response to interferon-gamma [IDA]
- cytokine-mediated signaling pathway [TAS]
- immune response [NAS]
- interferon-gamma-mediated signaling pathway [TAS]
- positive regulation of T cell activation [IMP]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interferon-gamma production [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle membrane [TAS]
- Golgi membrane [TAS]
- MHC class II protein complex [IDA]
- cell surface [IMP]
- clathrin-coated endocytic vesicle membrane [TAS]
- endocytic vesicle membrane [TAS]
- integral component of lumenal side of endoplasmic reticulum membrane [TAS]
- integral component of plasma membrane [NAS]
- lysosomal membrane [TAS]
- plasma membrane [TAS]
- trans-Golgi network membrane [TAS]
- transport vesicle membrane [TAS]
Homo sapiens
PREY
EPHA4
HEK8, SEK, TYRO1
EPH receptor A4
GO Process (13)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- corticospinal tract morphogenesis [ISS]
- fasciculation of motor neuron axon [ISS]
- fasciculation of sensory neuron axon [ISS]
- motor neuron axon guidance [ISS]
- negative regulation of axon regeneration [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of Rho guanyl-nucleotide exchange factor activity [IDA]
- protein autophosphorylation [IDA]
- regulation of Rac GTPase activity [ISS]
- regulation of Rap GTPase activity [ISS]
- regulation of astrocyte differentiation [ISS]
- regulation of axonogenesis [ISS]
- regulation of dendritic spine morphogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Cell Jul. 16, 2015; 162(2);425-40 [Pubmed: 26186194]
Quantitative Score
- 0.947651494 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.947651494, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Curated By
- BioGRID