An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Transcription factor interaction with COMPASS-like complex regulates histone H3K4 trimethylation for specific gene expression in plants.

Song ZT, Sun L, Lu SJ, Tian Y, Ding Y, Liu JX

Accumulation of unfolded or misfolded proteins causes endoplasmic reticulum (ER) stress, which activates a set of ER membrane-associated transcription factors for protein homeostasis regulation. Previous genome-wide chromatin immunoprecipitation analysis shows a strong correlation between histone H3K4 trimethylation (H3K4me3) and active gene expression. However, how the histone modification complex is specifically and timely recruited to the active promoters remains unknown. Using ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 03, 2015; 112(9);2900-5 [Pubmed: 25730865]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BZIP60

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]sequence-specific DNA binding transcription factor activity [IDA, ISS]

Gene Ontology Cellular Component

TRO