SIZ1
Gene Ontology Biological Process
- cell division [IMP]
- cell growth [IMP]
- cellular response to phosphate starvation [IMP]
- detection of phosphate ion [IDA]
- developmental growth [IMP]
- embryo sac development [IMP]
- heat acclimation [IMP]
- induced systemic resistance, jasmonic acid mediated signaling pathway [IMP]
- negative regulation of flower development [IMP]
- negative regulation of systemic acquired resistance [IMP]
- ovule development [IMP]
- pollen tube guidance [IMP]
- protein sumoylation [IDA]
- regulation of abscisic acid-activated signaling pathway [IMP]
- regulation of growth [IMP]
- regulation of nitrate assimilation [IMP]
- regulation of response to water deprivation [IMP]
- regulation of salicylic acid metabolic process [IMP]
- response to freezing [IMP]
- response to water deprivation [IMP]
Gene Ontology Molecular Function
FLC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Sumoylation)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
FLC-mediated flowering repression is positively regulated by sumoylation.
Flowering locus C (FLC), a floral repressor, is a critical factor for the transition from the vegetative to the reproductive phase. Here, the mechanisms regulating the activity and stability of the FLC protein were investigated. Bimolecular fluorescence complementation and in vitro pull-down analyses showed that FLC interacts with the E3 small ubiquitin-like modifier (SUMO) ligase AtSIZ1, suggesting that AtSIZ1 is ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIZ1 FLC | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 1239044 | |
| SIZ1 FLC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 1239045 |
Curated By
- BioGRID