An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Two distinct families of protein kinases are required for plant growth under high external Mg2+ concentrations in Arabidopsis.

Mogami J, Fujita Y, Yoshida T, Tsukiori Y, Nakagami H, Nomura Y, Fujiwara T, Nishida S, Yanagisawa S, Ishida T, Takahashi F, Morimoto K, Kidokoro S, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K

Protein phosphorylation events play key roles in maintaining cellular ion homeostasis in higher plants, and the regulatory roles of these events in Na(+) and K(+) transport have been studied extensively. However, the regulatory mechanisms governing Mg(2+) transport and homeostasis in higher plants remain poorly understood, despite the vital roles of Mg(2+) in cellular function. A member of subclass III sucrose ... [more]

Plant Physiol. Mar. 01, 2015; 167(3);1039-57 [Pubmed: 25614064]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT1G78290 AT5G21326	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Mogami J (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT1G78290

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [ISS]

Gene Ontology Cellular Component

AT5G21326