An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Two distinct families of protein kinases are required for plant growth under high external Mg2+ concentrations in Arabidopsis.

Mogami J, Fujita Y, Yoshida T, Tsukiori Y, Nakagami H, Nomura Y, Fujiwara T, Nishida S, Yanagisawa S, Ishida T, Takahashi F, Morimoto K, Kidokoro S, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K

Protein phosphorylation events play key roles in maintaining cellular ion homeostasis in higher plants, and the regulatory roles of these events in Na(+) and K(+) transport have been studied extensively. However, the regulatory mechanisms governing Mg(2+) transport and homeostasis in higher plants remain poorly understood, despite the vital roles of Mg(2+) in cellular function. A member of subclass III sucrose ... [more]

Plant Physiol. Mar. 01, 2015; 167(3);1039-57 [Pubmed: 25614064]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNRK2.2 AT5G21326	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Mogami J (2015)	Low	-	BioGRID	-
SNRK2.2 AT5G21326	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mogami J (2015)	Low	-	BioGRID	-
SNRK2.2 AT5G21326	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Mogami J (2015)	Low	-	BioGRID	1239085
SNRK2.2 AT5G21326	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Mogami J (2015)	Low	-	BioGRID	1239078

Curated By

BioGRID

Interaction Summary

AT5G21326

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [ISS]protein kinase activity [ISS]

Gene Ontology Cellular Component

SNRK2.2

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [IDA, ISS]protein binding [IPI]protein kinase activity [IDA]