BAIT

RBOHD

ATRBOHD, MCA23.25, MCA23_25, RESPIRATORY BURST OXIDASE, respiratory burst oxidase homologue D, AT5G47910
respiratory burst oxidase-D
Arabidopsis thaliana (Columbia)
PREY

BAK1

ATBAK1, ATSERK3, BRI1-associated receptor kinase, ELG, ELONGATED, F17M5.190, F17M5_190, RECEPTOR KINASES LIKE SERK 10, RKS10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, AT4G33430
Leu-rich receptor Serine/threonine protein kinase BAK1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity.

Kadota Y, Sklenar J, Derbyshire P, Stransfeld L, Asai S, Ntoukakis V, Jones JD, Shirasu K, Menke F, Jones A, Zipfel C

The rapid production of reactive oxygen species (ROS) burst is a conserved signaling output in immunity across kingdoms. In plants, perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors (PRRs) activates the NADPH oxidase RBOHD by hitherto unknown mechanisms. Here, we show that RBOHD exists in complex with the receptor kinases EFR and FLS2, which are the PRRs ... [more]

Mol. Cell Apr. 10, 2014; 54(1);43-55 [Pubmed: 24630626]

Throughput

  • Low Throughput

Curated By

  • BioGRID