An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Arabidopsis OR proteins are the major posttranscriptional regulators of phytoene synthase in controlling carotenoid biosynthesis.

Zhou X, Welsch R, Yang Y, Alvarez D, Riediger M, Yuan H, Fish T, Liu J, Thannhauser TW, Li L

Carotenoids are indispensable natural pigments to plants and humans. Phytoene synthase (PSY), the rate-limiting enzyme in the carotenoid biosynthetic pathway, and ORANGE (OR), a regulator of chromoplast differentiation and enhancer of carotenoid biosynthesis, represent two key proteins that control carotenoid biosynthesis and accumulation in plants. However, little is known about the mechanisms underlying their posttranscriptional regulation. Here we report that ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 17, 2015; 112(11);3558-63 [Pubmed: 25675505]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT5G06130 PSY	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zhou X (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT5G06130

Gene Ontology Cellular Component

PSY

Gene Ontology Biological Process

Gene Ontology Molecular Function

geranylgeranyl-diphosphate geranylgeranyltransferase activity [ISS, TAS]phytoene synthase activity [TAS]