BAIT

FD

FD-1, T19K4.30, atbzip14, AT4G35900
Basic-leucine zipper (bZIP) transcription factor family protein
Arabidopsis thaliana (Columbia)
PREY

CPK6

ARABIDOPSIS THALIANA CALCIUM-DEPENDENT PROTEIN KINASE 6, ARABIDOPSIS THALIANA CALMODULIN-DOMAIN PROTEIN KINASE 3, ATCDPK3, ATCPK6, CALCIUM-DEPENDENT PROTEIN KINASE 6, CALMODULIN-DOMAIN PROTEIN KINASE 3, F5J6.13, F5J6_13, calcium dependent protein kinase 6, AT2G17290
calcium dependent protein kinase 6
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Calcium-dependent protein kinases responsible for the phosphorylation of a bZIP transcription factor FD crucial for the florigen complex formation.

Kawamoto N, Sasabe M, Endo M, Machida Y, Araki T

Appropriate timing of flowering is critical for reproductive success and necessarily involves complex genetic regulatory networks. A mobile floral signal, called florigen, is a key molecule in this process, and flowering locus T (FT) protein is its major component in Arabidopsis. FT is produced in leaves, but promotes the floral transition in the shoot apex, where it forms a complex ... [more]

Sci Rep Feb. 11, 2015; 5(0);8341 [Pubmed: 25661797]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CPK6 FD
Biochemical Activity
Biochemical Activity

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Low-BioGRID
1240454

Curated By

  • BioGRID